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HPLC Guide

Posted by dapo on Jun 12, 2009 in Talking point

I am currently working in a section with lots of HPLC equipment.  We have recently had the pleasure of several work experience people come and visit our lab and I was asked to show one of these people what I was doing.  I was in the middle of prepearing to do a batch of vitamins A & E for analysis. 

I explain what I was doing during the preperation, then I described the equipment I was about to use to perform the analysis, a HPLC system.  I pointed out the basic elements of the system, pump, column etc and I then pointed out the same elements in the other HPLC systems in the section, including the same elements in the tandem mass spec.

Thinking about this experience reminded me about a picture I had prepared for training purposes of a HPLC system with labels pointing out the various elements.  I thought this image would be useful for other people to use for training purposes also.  I have included a labelled and unlabelled version for people to download and use.

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